Tønjum Gruppe - Molecular Microbiology - Rikshospitalet - UiO

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Restriction Enzyme Buffers

Fermentas Five Buffer System	NEB
1X Buffer B (blue) 10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl₂, 0.1 mg/ml BSA *1X Buffer SacI:* 10 mM Bis-Tris Propane-HCl (pH 6.5 at 37°C) 10 mM MgCl₂ and 0.1 mg/ml BSA.	1X NEBuffer 1: 10 mM Bis-Tris-Propane-HCl 10 mM MgCl₂ 1 mM Dithiothreitol pH 7.0 @ 25°C
1X Buffer G (green) 10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl₂, 50 mM NaCl, 0.1 mg/ml BSA	1X NEBuffer 2: 50 mM NaCl 10 mM Tris-HCl 10 mM MgCl₂ 1 mM Dithiothreitol pH 7.9 @ 25°C
1X Buffer O (orange) 50 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl₂, 100 mM NaCl, 0.1 mg/ml BSA	1X NEBuffer 3: 100 mM NaCl 50 mM Tris-HCl 10 mM MgCl₂ 1 mM Dithiothreitol pH 7.9 @ 25°C
1X Buffer R (red) 10 mM Tris-HCl (pH 8.5 at 37°C), 10 mM MgCl₂, 100 mM KCl, 0.1 mg/ml BSA	1X NEBuffer 4: 50 mM potassium acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 1 mM Dithiothreitol pH 7.9 @ 25°C
1X Buffer Tango™ (yellow) 33 mM Tris-acetate (pH 7.9 at 37°C), 10 mM magnesium acetate, 66 mM potassium acetate, 0.1 mg/ml BSA	Promega MULTI-CORE™ Buffer (1X): 25mM Tris-Acetate pH 7.5 (at 37°C) 100mM potassium acetate 10mM magnesium acetate 1mM DTT.
1X Buffer for BamHI 10 mM Tris-HCl (pH 8.0 at 37°C), 5 mM MgCl₂, 100 mM KCl, 0.02% Triton X-100, 1 mM 2-mercapthoethanol, 0.1 mg/ml BSA	1X NEBuffer 3: 100 mM NaCl 50 mM Tris-HCl 10 mM MgCl₂ 1 mM Dithiothreitol pH 7.9 @ 25°C
1X Buffer for EcoRI 50 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl₂, 100 mM NaCl, 0.02% Triton X-100, 0.1 mg/ml BSA	1X NEBuffer EcoRI: 100 mM Tris-HCl 50 mM NaCl 10 mM MgCl₂ 0.025 % Triton X-100 pH 7.5 @ 25°C

Promega 4-CORE^® Buffer System

Restriction Enzymes Resource

Composition of Promega Restriction Enzyme Reaction Buffers (1X)

Buffer	pH (at 37°C)	Tris-HCl (mM)	MgCl₂ (mM)	NaCl (mM)	KCl (mM)	DTT (mM)
A	7.5	6	6	6	-	1
B	7.5	6	6	50	-	1
C	7.9	10	10	50	-	1
D	7.9	6	6	150	-	1
E	7.5	6	6	100	-	1
F	8.5	10	10	100	-	1
G	8.2	50	5	-	-	-
H	7.5	90	10	50	-	-
J	7.5	10	7	-	50	1
K	7.4	10	10	-	150	-
L	9.0	10	3	100	-	-

MULTI-CORE™ Buffer (1X) = 25mM Tris-Acetate, pH 7.5 (at 37°C), 100mM potassium acetate, 10mM magnesium acetate, 1mM DTT.

Notes:

For each 10°C rise in temperature between 0°C and 25°C, the pH of Tris buffers decreases 0.31 pH units.
For each 10°C rise in temperature between 25°C and 37°C, the pH of Tris buffers decreases 0.25 pH units.

Restriction Enzyme Buffers

Fermentas

NEB

Promega

Promega 4-CORE® Buffer System

Restriction Enzymes Resource

Promega 4-CORE^® Buffer System