 Techniques  The LMN possesses competence and access to the necessary equipment for a broad range of advanced methods and technologies.
Immunocytochemistry:
Generation, purification, and testing of primary antibodies
Gold-, enzyme- and fluorescent labeling of secondary antibodies
In situ hybridization
Light- and electron microscopy
Electron microscopy:
Transmission electron microscopy (TEM)
Freeze-substitution embedding
Ultracryotomy
Light microscopy:
Visual light microscopy
IR-DIC video microscopy
Fluorescence microscopy with cooled CCD camera, Ca2+ imaging
Cryostats, Vibratomes
Digital imaging:
On-line image-acquisition in wide field light- and electron microscopy
Networked image-storage, -processing, -analysis.
Semi-automated counting of particle density over organelles and membranes, data automatically aggregated and exported for graphical and statistical analysis
Video-cameras, standard and IR-sensitive
High-resolution, cooled, slow-scan CCD-cameras for fluorescence imaging
Image-combining computerized microscopy
3D-imaging: see Laser-scan microscopy
Laserscan and multimode microscopy (LSM laboratory):
Leica TCS SP scanner with three microscopes on vibration-free optical table
Confocal and multi-photon excitation modes. Mirror-coupled Titanium Sapphire laser for multi photon excitation mode, including uncaging
Co-ordinated imaging and electrophysiology of thick and thin specimens/single cells (upright fixed stage, respectively inverted, microscope)
Full complements of objectives, fluorescence filters for widefield mode, ports for CCD- and videocameras
Temperature-controlled perfusion system
Cell and tissue cultures:
Primary cell culture
Cell transfection
Dissociated neuronal cultures
Organotypic slice cultures |
